Domani Jackson attended Mater Dei High School in Santa Ana, California where he played football and competed in track and field. In 2021, he tied the California state record in the 100 meters with a time of 10.25 seconds and was selected to the PrepStar Dream Team after helping Mater Dei to a 12-0 record adn the 2021 national, state open Division and CIF Southern Division I titles. He was ranked by ESPN at No. 9 nationally and No. 1 in California in its ranking of the top college recruits in the Class of 2022. He was a consensus five-star recruit and after narrowing his final choices to USC, Michigan and Alabama, Jackson committed to USC in December 2021.
Jackson played sparingly in seven games for USC in 2022. He totaled two tackles and one pass defended. He saw some playing time in different nickel packages in his sophomore season and ended the season with 33 tackles and three passes defended in 11 games.
After the season Jackson entered the transfer portal and elected to transfer to the University of Alabama for the 2024 football season.